en Neuroscience Research <div style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:200%"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:12.0pt"><span style="line-height:200%">Background and Aim:</span></span></b><span style="font-size:12.0pt"><span style="line-height:200%"> Epilepsy as a neurological disorder is characterized by unpredictable and periodic seizures. In the present study, the anticonvulsant effect of different doses of salep hydroalcoholic extract on pentylenetetrazole (PTZ) induced seizures was investigated.</span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:200%"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:12.0pt"><span style="line-height:200%">Methods and Materials/Patients</span></span></b><span style="font-size:12.0pt"><span style="line-height:200%">: The animal model of seizure was established by the intraperitoneal injection of pentylenetetrazole (PTZ- 85 mg/kg). Hydroalcoholic extract of salep was administered to the animals in 3 doses (80, 160, and 320 mg/kg). </span></span></span></span></span></span><span style="font-size:11pt"><span style="line-height:200%"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span style="font-size:12.0pt"><span style="line-height:200%">The parameters of the onset and duration of tonic-clonic seizure, total seizure time, balance (falling), and jumping during seizure mortality rate were measured. The results were analyzed by ANOVA test at a significant level of p&le;0.05.</span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:200%"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:12.0pt"><span style="line-height:200%">Results</span></span></b><span style="font-size:12.0pt"><span style="line-height:200%">: The results of the current study indicated that salep extract increased the delay in the onset of seizure in the PTZ + extract group in comparison with the PTZ group. The duration of tonic-clonic seizure and the number of falling, jumping, and total seizure times were significantly decreased by salep extract compared to the PTZ group. In the seizure + salep extract, the number and percentage of 24-hour mortality among animals decreased with increasing dosage. All changes were dose-dependent and 320 mg/kg showed the most effect.</span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:200%"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:12.0pt"><span style="line-height:200%">Conclusion</span></span></b><span style="font-size:12.0pt"><span style="line-height:200%">:&nbsp; </span></span><span lang="EN-GB" style="font-size:12.0pt"><span style="line-height:200%">Extract of salep can probably have anticonvulsant properties, however, further studies are needed to clarify the exact mechanisms.</span></span></span></span></span></span></div> &nbsp; Epilepsy, Pentylenetetrazole, Male rats, Extract of Salep, Tonic colonic 1 9 http://irjns.org/browse.php?a_code=A-10-512-1&slc_lang=en&sid=1 Hossein Kargar Jahromi 100319475328460010796 100319475328460010796 No Research Center for Non-Communicable Diseases, Jahrom University of Medical Sciences, Jahrom, Iran Arman Khorram 100319475328460010797 100319475328460010797 No Student Research Committee, Jahrom University of Medical Sciences, Jahrom, Iran Shekofeh Atashpoor 100319475328460010798 100319475328460010798 No Department of Pharmacology, Faculty of Medicine, Jahrom University of Medical Sciences, Jahrom, Iran Maryam Jalali 100319475328460010799 100319475328460010799 No Research Center for Social Determinants of Health, Jahrom University of Medical Sciences, Jahrom, Iran Zahra Kargar Jahromi 100319475328460010800 100319475328460010800 No Research Center for Non-Communicable Diseases, Jahrom University of Medical Sciences, Jahrom, Iran Fatemeh Mohammadi 100319475328460010801 100319475328460010801 No Department of Basic Sciences, Sirjan School of Medical Sciences, Sirjan, Iran Mohammad Amin Serpoosh 100319475328460010802 100319475328460010802 No Department of Basic Sciences, Sirjan School of Medical Sciences, Sirjan, Iran Najmeh Sadeghi najmehsa@yahoo.com 100319475328460010803 100319475328460010803 Yes Department of Basic Sciences, Sirjan School of Medical Sciences, Sirjan, Iran